Determination of crude fiber content in sugar cane

Determination of crude fiber content in sugar cane

As we all know, sugar cane is a raw material for making sucrose. It belongs to the genus Saccharum and is a temperate and tropical crop. The world's largest sugarcane producer is located in Brazil in South America, followed by India and China. In general, sugarcane needs to be planted in areas where the soil is fertile, where there is sufficient sunlight, and where there is a large temperature difference between winter and summer. Sugarcane grown in these areas contains higher sugar content. At the same time, sugarcane also contains a variety of vitamins, fats, proteins, organic acids, calcium, iron and other substances. It is a kind of fruit rich in nutrient plants.

This article describes how to determine crude fiber in sugar cane. Of course, if you want to determine the fat content in sugar cane, we can also use the instrument - crude fat analyzer to measure. However, this article focuses on the determination of crude fiber, so we will not discuss the determination of fat content in sugar cane.

Crude fibers in sugar cane, including cellulose, hemicellulose, lignin, and keratin are more complex. So if we use the instrument to determine, we can choose crude fiber analyzer, CXC-06 fiber analyzer, and if we want laboratory methods, then some of the following description will provide you with more practical methods.

First of all, sugar cane is a sample that is not easily smashed. The moisture content (B1) is determined according to GB 8858. At the same time, the sample was dried in an oven at 80° C., then crushed with a pulverizer and passed through a 0.84 mm sieve. After collection, put it in a jar for use.

Next, accurately weigh about 3g of the prepared sample and set it in a 500ml Erlenmeyer flask. At the same time, the water content (B2) was measured according to GB 8858.

After weighing, if the moisture content is high, most of the water can be evaporated in an oven at 80°C. If the fat content exceeds 1%, the sample can be soaked and washed with ether for several times, then the solvent is decanted and no residue can be lost. Finally, the excess solvent is dried. The next step is acid treatment: (1) Add 200ml of sulfuric acid solution at 95~100°C (measure at room temperature). If there is more foaming, add a few drops of defoamer in advance. Put on the condenser tube, immediately heated to a slight boiling (about 2min) on a hot plate, slightly boiling 30 persons 1min. During the micro-boiling process, the Erlenmeyer flask should be shaken several times to prevent the sample from sticking on the liquid surface. (2) Connection number suction filter device, quickly filtered and separated. And the residue was washed with hot water of 95~100°C until the filtrate was neutral (blue litmus paper does not change color). Followed by alkali treatment: (1) The residue of linen placed on the inner wall of the short neck funnel, do not block the water outlet. The residue was rinsed into the original conical flask with 200 ml of sodium hydroxide solution (measured at room temperature) of 95-100°C. Put on the condenser tube, immediately heated to a slight boiling (about 2min) on a hot plate, slightly boiling 30 persons 1min. (2) Connect the suction filter unit (courtesan cockroach with asbestos) and quickly separate it by suction filtration. The residue was washed with 20 ml of sulfuric acid solution (room temperature), washed thoroughly with hot water of 95 to 100°C, and then drained, followed by washing once with ethanol and ether (degreasing sample was not washed with ether), and the mixture was drained.

Then dry and ashing process. Drying: The residue is placed in a temperature range of 130±2°C and dried in a drying oven for 2 h. After being cooled to room temperature in a desiccator, it is weighed (A1). Ashing: Ashes containing the residue in a 550-person 250-degree muffle furnace are ashed for 2 hours. When the temperature drops below 200 DEG C, they are taken out of the desiccator and cooled to room temperature and weighed. Then put the Ashes in the muffle furnace for 1h. Repeat until the difference of 2 weighings does not exceed 0.5 mg (A2).

The last step is the calculation.

In the formula: A1 - Gushi 坩埚 + coarse fiber + residue ash, g;

A2 - Old Ashes + residue ash, g;

W - mass of sample, g.

B1 - Moisture content determined by section 6.2;

B2 - Moisture content determined according to 7.1.2.

The arithmetic average of the two measurements was taken as the measurement result. When the measurement result is less than 10%, the absolute value of the difference between the two measurement results must not exceed 0.4; when the measurement result is greater than 10%, the relative value of the difference between the two results must not exceed 4%.